![]() ![]() Rapid Commun Mass Spectrom 23:3843–3854Ĭai H, Gu X, Scanlan MS, Ramatlapeng DH, Lively CR (2012) Real-time PCR assays for detection and quantification of porcine and bovine DNA in gelatin mixtures and gelatin capsules. īuckley M, Collins M, Thomas-Oates J, Wilson JC (2009) Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Analyst 134(5):966–972Īzira TN, Man YBC, Hafidz RNRM, Aina MA, Amin I (2014) Use of principal component analysis for differentiation of gelatine sources based on polypeptide molecular weights. Overall, LAMP assay in this study showed an excellent specificity, sensitivity and rapidity in detection of porcine DNA in gelatin products.Īhmed MU, Saito M, Hossain MM, Rao SR, Furui S, Hino A, Takamura Y, Takagi M, Tamiya E (2009) Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplification. Out of these five positive samples, three were not labeled containing porcine gelatin. Analysis against 32 samples of gelatin products showed that five samples were found to contain porcine DNA two samples out of six gelatin powder samples and three gelatin capsule samples out of nine. ![]() The analytical sensitivity of the LAMP assay for porcine DNA detection is 1 pg/µL using both GENIE (within 30 m) and MYRM (within 60 m) reaction mixtures. The porcine-specific primers were shown to be specific only to Sus scrofa against 14 DNA of other meat species. ![]() Here we used two different reaction mixtures for LAMP assay (GENIE and MYRM) against the same DNA samples extracted from gelatin products and porcine-specific primers to detect the presence of porcine DNA. The porcine-specific primers were designed according to mitochondrial DNA of Cytochrome b gene sequence. We documented here a porcine-specific loop-mediated isothermal amplification (LAMP) to identify porcine traces in gelatin products. Low DNA concentration recovered from highly processed products such as gelatin and gelatin-based products renders difficulty in detecting porcine contamination using conventional PCR techniques. ![]()
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